WebThe goal of the protocols described here is to measure the affinity of a ligand for cell surface receptor(s) and to determine the number of receptors per cell, following the classic method of Scatchard [1]. The preferred method for analysis of ligand binding is to... WebMathematically, the Scatchard equation is related to Eadie-Hofstee method, which is used to infer kinetic properties from enzyme reaction data. Many modern methods for measuring binding such as surface plasmon resonance and isothermal titration calorimetry provide …
Scatchard Equation - an overview ScienceDirect Topics
WebThe osmotic and activity coefficients of aqueous mixtures of NaCl and MgCl/sub 2/ have been determined at 25/sup 0/C by using the isopiestic method. These measurements extend from low concentrations to the crystallization limits of the mixtures. They are critically compared to published isopiestic, direct vapor pressure, and emf data for this system. WebKeywords: binding radioligand radioligand binding Scatchard plot receptor binding competitive binding curve IC50 K d B max nonlinear regression curve Þtting ßuorescence INTRODUCTION A radioligand is a radioactively labeled drug that can associate with a receptor, trans-porter, enzyme, or any protein of interest. northern industrial sales whitehorse
Interaction of drugs with bovine and human serum albumin
WebEquilibrium dialysis is considered the reference method for measurement of free testosterone and is based on the passage of low molecular weight molecules through a membrane with a ... Using these parameters, a Scatchard plot was made, from which was obtained n, the number of binding sites per enzyme molecule and K A, the association ... WebThe Scatchard equation is an equation used in molecular biology to calculate the affinity and number of binding sites of a receptor for a ligand. It is named after the American chemist … WebNov 1, 1975 · Abstract. In saturation analysis the Scatchard plot is a generally accepted method for calculation of the affinity constant, K, and the molar concentration, q, of the binder.However, in a system where the K's for the labeled and unlabeled ligand are unequal, a nonlinear plot can be obtained from which incorrect values for K and q may be calculated. northern industrial sales terrace bc