WebNov 1, 2012 · In this study, aqueous extracts of peels of best known citrus fruits namely grapefruit (Citrus paradisi), lemon (C. limon), lime (C. aurantifolia) and orange (C. sinensis) were used. Depending on polyphenols content, the extracts were graded as orange > lemon > lime > grapefruit. Effects of the extracts on the release of histamine from rat peritoneal … Rat peritoneal exudate cells (PECs; 500 μL, 1 × 106) were suspended in 48 μL of 25 mM CaCl2, 12 μL of various concentrations of samples, and/or 120 μL of a 5 μM A23187 solution, and then the volume was adjusted to 1.2 mL with tyrode buffer, and incubated for 20 min at 37 °C. The reaction was terminated by … See more Folin-Ciocalteu’s phenol reagent, fish gelatin, and histamine dihydrochloride were purchased from Sigma-Aldrich Co (St. Louis, MO). A-23187, bovine serum albumin (BSA), and … See more Grapefruit (Citrus paradisi Mackfad.), lemon (C. limon (L.) Burm. f.), lime (C. aurantifolia Christm.) and orange (C. sinensis(L.) Osbeck) were purchased from local markets at … See more The total concentration of phenolics in the extracts was determined according to the Folin-Ciocalteu method (Vinson et al. 2001) with gallic acid (GA) as the standard and expressed (mM) as gallic acid equivalents (GAE) of … See more Male SD rats (8 weeks old) were used for the preparation of peritoneal exudates cells to conduct inhibition of histamine release by the extracts whereas male ddY mice (5–6 weeks old; … See more
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