WebOct 31, 2024 · Conversely, two subsequent independent studies Su et al., 2024) reported on the cloning of Fhb1 and postulated a histidine-rich calcium-binding (TaHRC or His) gene as the Fhb1 candidate. WebJul 13, 2024 · Fusarium head blight (FHB) and stem rust are among the most devastating diseases of wheat worldwide. Fhb1 is the most widely utilized and the only isolated gene for FHB resistance, while Sr2 is a …
A deletion mutation in TaHRC confers Fhb1 resistance to …
WebFhb1 is the most consistently reported quantitative trait locus (QTL) for FHB resistance breeding. Here we report the map-based cloning of Fhb1 from a Chinese wheat cultivar Sumai 3. By mutation analysis, gene silencing and transgenic overexpression, we show that a pore-forming toxin-like (PFT) gene at Fhb1 confers FHB resistance. WebTo obtain wheat germplasm containing both Fhb1 and Sr2 genes, we developed and screened two F 2 populations using tightly linked DNA markers, Xgwm533 and csSr2 for Sr2, and UMN10, and a newly designed marker UMN10 v2 for Fhb1. Among 1949 F 2 plants, 43 were identified with Fhb1 and Sr2 in coupling phase. After screened with … twin mice
Development and validation of diagnostic markers for Fhb1 …
WebAug 22, 2024 · Key message Functional markers were developed based on the critical sequence deletion of TaHRC in the Fhb1 region and validated to be diagnostic in a worldwide wheat collection. Abstract Wheat Fusarium head blight (FHB) is a devastating disease in wheat and barley worldwide. Growing FHB-resistant cultivars is an effective … WebJul 14, 2024 · The lab of Ma previously cloned Fhb1, which inhibits FHB spread within spikes, and fine mapped Fhb4 and Fhb5, which condition resistance to initial infection of Fusarium spp., from FHB-resistant indigenous line Wangshuibai (WSB). In this study, these three QTLs were simultaneously introduced into five modern Chinese wheat cultivars or … WebIt thus appeared that the WSB/S3 His allele (His R ) was the sole Fhb1 candidate. This was further supported by RNA sequencing (RNA-Seq) data generated with Fusarium-infected WSB tissues, in which only His R was matched with numerous reads, with 10.7 fragments per kilobase of transcript per million mapped reads on average. taipei medical university swimming pool